LDH-C4 in human seminal plasma and testicular function.
نویسندگان
چکیده
منابع مشابه
A further study of human seminal plasma lactate dehydrogenase-C4 (LDH-C4): kinetic properties of LDH-C4.
Lactate dehydrogenase-C4 (LDH-C4) from human seminal plasma was purified by 40-60% (NH4)2SO4 precipitation, Oxamate-Sepharose 4B affinity column chromatography and AMP affinity column chromatography. Enzyme kinetic studies of human seminal plasma LDH-C4 were performed with purified LDH -C4. The human LDH-C4 exhibits a much higher affinity for pyruvate and alpha-ketovalerate than lactate. Appare...
متن کاملA Further Study of Human Seminal Plasma Lactate Dehydrogenase-c Cldh-c4): Kinetic Properties of Ldh-c4
Lactate dehydrogenase.C. (LDH-C.) from human seminal plasma was purified by 40~60% (NH.)2S0. precipitation, Oxamate-Sepharose 4B affinity column chromatography and AMP affinity column chromatography. Enzyme kinetic studies of human seminal plasma LDH-C, were performed with purified LDH-C.. The human LDH-C. exhibits a much higher affinity for pyruvate and a-ketovalerate than lactate. Apparent Km...
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The aim of the present study was to determine seasonal variations in semen characteristics, testicular size, and plasma testosterone concentration in Iranian Khalkhali goats. An artificial vagina was used to collect semen from 10 bucks (2–4 years of age), from November 2013 to October 2014. Testicular circumference was higher (P<0.05) from September to October. Ejaculate volume, sperm ...
متن کاملEvaluation of LDH Activity and Its Relationship with Fructose Levels in Seminal Plasma of Normospermic and Asthenospermic Males
ABSTRACT Background and Objectives: sperm motility mainly depends on aerobic glycolysis, and lactate dehydrogenase (LDH) is a key enzyme in the last step of this process. On the other hand, fructose is considered as the main energy source for sperm motility. Therefore, this study aimed to evaluate the LDH activity and fructose levels and their cor...
متن کاملMicromethod for determination of lactate dehydrogenase isoenzyme C4 activity in human seminal plasma.
We have developed an automated enzymatic assay for LDH-C4, a lactate dehydrogenase (EC 1.1.1.27) isoenzyme found exclusively in spermatozoa, as a marker of human spermatogenesis. LDH-C4 activity is assayed bichromatically in the Abbott ABA-100 spectrophotometer, with 2-oxohexanoate as substrate. As many as 27 samples (2.5 microL) of human seminal plasma can be analyzed sequentially, with appare...
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ژورنال
عنوان ژورنال: International Journal of Andrology
سال: 1985
ISSN: 0105-6263,1365-2605
DOI: 10.1111/j.1365-2605.1985.tb00850.x